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Jianghan University

4 ARTICLES PUBLISHED IN JoVE

Medicine

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System

Wusong Zou¹, Yanqun Yang¹, Ye Gu¹, Pengfei Zhu¹, Mingjing Zhang¹, Zhong Cheng¹, Xiaoyan Liu¹, Yijun Yu¹, Xiaohong Peng¹

¹Heart Center at Puai Hospital, Wuhan Puai Hospital, Jianghan University

Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.

Behavior

Modified Blood Collection from Tail Veins of Non-anesthetized Mice with a Vacuum Blood Collection System and Eyeglass Magnifier

Xiaoyan Liu^*1, Huanhuan Li^*1, Jie Wu¹, Yijun Yu¹, Mingjing Zhang¹, Wusong Zou¹, Ye Gu¹

¹Heart Center, Wuhan Fourth Hospital, Puai Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology

This study reports blood sampling from tail vein in mice using a vacuum extraction tube system with eyeglass magnifier. Our method is easy to practice and could be used for repeat blood sampling in mice.

Neuroscience

Induction of Acute Ischemic Stroke in Mice Using the Distal Middle Artery Occlusion Technique

Changlong Leng^*1, Youwei Li^*1, Yaojian Sun¹, Wei Liu¹

¹Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University

Here, we present a protocol to establish a distal middle cerebral artery occlusion (dMCAO) model through transcranial electrocoagulation in C57BL/6J mice and evaluate the subsequent neurological behavior and histopathological features.

Medicine

A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia

Yaojian Sun^*1, Changlong Leng^*1, Kang Ma¹, Wei Liu¹

¹Hubei Key Laboratory of Cognitive and Affective Disorder, Institute of Biomedical Sciences, School of Medicine, Jianghan University

This study presents a protocol for establishing a highly reproducible animal model of hemorrhagic transformation (HT) using middle cerebral artery occlusion/reperfusion (MCAO/R) in C57BL/6 mice with acute hyperglycemia.