Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated SamplesYan Wei Lim 1, Matthew Haynes 2, Mike Furlan 1, Charles E. Robertson 3, J. Kirk Harris 4, Forest Rohwer 1
1Department of Biology, San Diego State University, 2DOE Joint Genome Institute, 3Department of Molecular, Cellular and Developmental Biology, University of Colorado, 4Department of Pediatrics, School of Medicine, University of Colorado
Using the cystic fibrosis airway as an example, the manuscript presents a comprehensive workflow comprising a combination of metagenomic and metatranscriptomic approaches to characterize the microbial and viral communities in animal-associated samples.