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National University Health System

3 ARTICLES PUBLISHED IN JoVE

Medicine

Victor T.T. Chan¹, Tiffany H.K. Tso¹, Fangyao Tang¹, Clement Tham¹, Vincent Mok^2,3,4, Christopher Chen^5,6, Tien Y. Wong^7,8, Carol Y. Cheung¹

¹Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, ²Department of Medicine & Therapeutics, The Chinese University of Hong Kong, ³Therese Pei Fong Chow Research Centre for Prevention of Dementia, The Chinese University of Hong Kong, ⁴Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, ⁵Memory Aging and Cognition Centre, National University Health System, ⁶Department of Pharmacology, National University of Singapore, ⁷Singapore Eye Research Institute, Singapore National Eye Centre, ⁸Duke-NUS Medical School, National University of Singapore

The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.

Immunology and Infection

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Guo Hong^*1,2, Shuangyi Fan^*3, The Phyu³, Priyanka Maheshwari², Michal Marek Hoppe², Hoang Mai Phuong², Sanjay de Mel⁴, Michelle Poon⁴, Siok-Bian Ng^2,3, Anand D. Jeyasekharan^2,4

¹Department of Laboratory Medicine, AnSteel Group General Hospital, ²Cancer Science Institute of Singapore, National University of Singapore, ³Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, ⁴Department of Haematology-Oncology, National University Health System

Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

Immunology and Infection

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Zhe Zhang Ryan Lew^*1, Jing Liu^*1, Hsiao Hui Ong¹, Vivian Jiayi Tan², Annika Luukkainen³, Yew Kwang Ong^1,4, Mark Thong^1,4, Kia Joo Puan⁵, Vincent Tak Kwong Chow^2,6, Kai Sen Tan^1,2,6, De Yun Wang^1,6

¹Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, ²Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ³Haartman Institute, Medicum, University of Helsinki, ⁴Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, ⁵Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), ⁶NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses.