Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular VesiclesNicole Comfort 1, Kunheng Cai 2, Tessa R. Bloomquist 1, Madeleine D. Strait 1, Anthony W. Ferrante Jr. 2, Andrea A. Baccarelli 1
1Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, 2Department of Medicine, Naomi Berrie Diabetes Center, Columbia University
We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.