Particle Tracking Instrument Preparation and Nanoparticle Tracking Analysis
4:01
NTA Data Processing, Display, and Interpretation
5:29
Cuvette Cleaning
6:33
Results: Using a Novel NTA Instrument to Estimate EV Size Distribution and Total Particle Concentration
8:33
Conclusion
Transcript
NTA results are very prone to operator bias. This protocol demonstrates the effects of altered NTA parameters on obtained results. A standardized method will help increase rigor and reproducibility in NTA analysis.
Analyzing the sample in a
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We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.