Hotchkiss Brain Institute at University of Calgary

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

Michael B. Keough¹, Samuel K. Jensen¹, V. Wee Yong^1,2

¹Department of Clinical Neurosciences, Hotchkiss Brain Institute at University of Calgary, ²Department of Oncology, Hotchkiss Brain Institute at University of Calgary

Demyelinating diseases can be modeled in animals by focal application of lysolecithin into the CNS. A single injection of lysolecithin into mouse spinal cord produces a lesion that spontaneously repairs over time. The goal is to study factors involved in de- and remyelination, and to test agents for enhancing repair.