Whole Mount Labeling of Cilia in the Main Olfactory System of MiceSonja Oberland 1,2, Eva Maria Neuhaus 1,2
1Pharmacology and Toxicology, Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin
Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.