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Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

DOI :

10.3791/52299-v

December 27th, 2014

December 27th, 2014

10,785 Views

1Pharmacology and Toxicology, Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

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Whole Mount Labeling

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