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University of California Riverside

3 ARTICLES PUBLISHED IN JoVE

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Biology

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Papri Chatterjee 1, Yuri Cheung 1, Chee Liew 1
1UCR Stem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

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Neuroscience

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Alison X. Xie 1, Kelli Lauderdale 1, Thomas Murphy 1, Timothy L. Myers 1, Todd A. Fiacco 2,3
1Graduate Program in Neuroscience, University of California Riverside, 2Department of Cell Biology and Neuroscience, University of California Riverside, 3Center for Glial-Neuronal Interactions, University of California Riverside

Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.

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Bioengineering

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
Deborah Pagliaccia *1,6, Douglas Hill *2, Emily Dang 1, Gerardo Uribe 1, Agustina De Francesco 1, Ryan Milton 2, Anthony De La Torre 2, Axel Mounkam 2, Tyler Dang 1, Sohrab Bodaghi 1, Irene Lavagi-Craddock 1, Alexandra Syed 1, William Grover 3, Adriann Okamba 4,5, Georgios Vidalakis *1
1Department of Microbiology and Plant Pathology, University of California Riverside, 2Technology Evolving Solutions (TES), 3Department of Bioengineering, University of California Riverside, 4Ecole Supérieure d'Ingénieurs Léonard de Vinci ESILV, 5University of California Riverside, 6California Agriculture and Food Enterprise (CAFÉ), University of California Riverside

We engineered, fabricated, and validated an instrument that rapidly processes phloem-rich bark citrus budwood tissues. Compared to current methods, the budwood tissue extractor (BTE) has increased sample throughput and decreased the required labor and equipment costs.

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