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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

DOI :

10.3791/3110-v

10:24 min

October 5th, 2011

October 5th, 2011

20,939 Views

1UCR Stem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

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Transfecting

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