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Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

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302 Views

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03:04 min

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June 30th, 2023

DOI :

10.3791/200348-v

June 30th, 2023

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Jiale Cheng¹^,², Jiong Zhu¹^,², Yujiao Liu²^,³, Chen Yang²^,³, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang¹^,²

¹School of Basic Medical Sciences, Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, ³School of Life Sciences, Hebei University

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Keywords Peptide Extraction

Small Extracellular Vesicles

Ultracentrifugation

Protease Inhibitor

Ultrasonic Crushing

Ultrafiltration

Trifluoroacetic Acid

Mass Spectrometry

Da série

Isolating Small Extracellular Vesicles and Extracting Their Peptidome

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