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Beijing Institute of Lifeomics

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences, Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, ³School of Life Sciences, Hebei University

This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.