Begin preparing the immunoglobulin A, or IgA nephropathy and control models after subjecting the mice to one week of adaptive rearing in a laboratory cage. Then for nine weeks, feed the model mice with 0.1%bovine gamma globulin, or BGG solution, in six millimolar aqueous hydrochloric acid. Next, inject 0.1 milliliters of 0.1%BGG solution in saline into the model animals'tail vein for three consecutive days while continuing the feeding as before.
After successfully preparing the IgA nephropathy model, on week nine, begin the dioscin or DIO intervention. To half of the mice from the model group selected as the DIO gavage group, feed appropriate drinks to the groups as demonstrated for eight weeks before proceeding with the subsequent analysis.
