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Ocular Tissue Embedding and Cryosectioning in Optimal Cutting Temperature Compound for Fluorescence Imaging

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63 Views

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02:29 min

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November 10th, 2023

DOI :

10.3791/201613-v

November 10th, 2023

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Erik A. Souverein¹, Aaron Nagiel²^,³^,⁴, Ksenia Gnedeva⁵^,⁶

¹Keck School of Medicine, University of Southern California, ²The Vision Center, Department of Surgery, Children’s Hospital Los Angeles, ³The Saban Research Institute, Children’s Hospital Los Angeles, ⁴USC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, ⁵Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, University of Southern California, ⁶Tina and Rick Caruso Department of Otolaryngology-Head and Neck Surgery, University of Southern California

Transcrição

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Ocular Tissue Embedding

Optimal Cutting Temperature Compound

Fluorescence Imaging

Cryo Protection

Sucrose Solution

Dissection Dish

Dissecting Microscope

Retinal Cryosection

Retinal Pigment Epithelium

Photoreceptor Layer

Bright field Image

Retinal Morphology

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