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Preparation of Single Cell Suspensions from Differentiated 3D Human Intestinal Organoids for Single Cell Transcriptomics

Please note that all translations are AI generated. Click here for the English version.

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02:22 min

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June 28th, 2024

DOI :

10.3791/201686-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcrição

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Single Cell Suspensions

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Enzymatic Cell Digestion

Cell Centrifugation

Trypan Blue Exclusion

Viable Cell Count

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Absorptive Enterocytes

Secretory Cells

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Integrating Single-Cell Transcriptomics with Human Intestinal Organoids

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