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Preparation of Single Cell Suspensions from Human Intestinal Organoids Differentiated as 96 Well Monolayers

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01:58 min

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June 28th, 2024

DOI :

10.3791/201688-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcrição

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Single Cell Suspension

Human Intestinal Organoids

Enzymatic Cell Digestion

Cell Culture Media

Cell Dissociation

Viable Cell Count

Micron Tip Strainer

Differentiation Medium

Incubator Conditions

Flow Through Collection

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