Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

0:06

Introduction

0:49

Dewaxing and Rehydrating Paraffin Sections

3:20

Unmasking Antigen with Heat-Induced Antigen Retrieval

5:14

Blocking Endogenous Peroxidase Activity and Nonspecific Binding of

8:17

Adding Primary and Secondary Antibodies

11:39

Preparing and Incubating Slides in the ABC Reagent

13:21

Preparing and Reacting Slides with DAB Substrate Reagent

16:10

Counterstaining and Mounting the Slides

20:46

Visualizing Anti-Tyrosine Hydroxylase Slides

21:07

Conclusion