Microscopy and Light Illumination at the Subcellular Level
7:07
Examining the Effect of RAC Activation on Local Ruffle Formation
9:29
Conclusion
A method for spatio-temporal control of small GTPase activity by light is described. This method is based on rapamycin-induced FKBP-FRB heterodimerization and photo-caging systems. Optimization of light-irradiation enables the spatio-temporally controlled activation of small GTPases at the subcellular level.