Human RBCs Isolation, Biotinylation and Osmolarity Adjustment
4:07
Preparation for Micropipettes and a Cell Chamber
6:24
BFP Experiment
10:25
Fluorescence BFP (FBFP) Experiment
11:51
Results: Representative T-cell Receptor and Peptide MHC Binding Kinetics Data & Calcium Fluorescence Data from the FBFP Experiment
13:14
Conclusion
We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.