June 1st, 2016
•This article describes a protocol for visualizing amyloid Aβ plaques in Alzheimer's disease mouse models using methoxy-X04, which crosses the blood-brain barrier and selectively binds to β-pleated sheets found in dense core Aβ plaques. It allows pre-screening of plaque-containing tissue sections prior to immunostaining and processing for electron microscopy.
Tags
Vídeos Relacionados
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
Serial Block-Face Scanning Electron Microscopy (SBEM) for the Study of Dendritic Spines
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados