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CHU de Québec Research Center

4 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
Julie Lessard 1, Julie Anne Côté 1, Marc Lapointe 1, Mélissa Pelletier 2, Mélanie Nadeau 1, Simon Marceau 1, Laurent Biertho 1, André Tchernof 1,2,3
1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University

Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

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Neuroscience

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
Kanchan Bisht *1, Hassan El Hajj *1, Julie C. Savage 1, Maria G. Sánchez 1, Marie-Ève Tremblay 1
1Neurosciences Axis, CHU de Québec Research Center

This article describes a protocol for visualizing amyloid Aβ plaques in Alzheimer's disease mouse models using methoxy-X04, which crosses the blood-brain barrier and selectively binds to β-pleated sheets found in dense core Aβ plaques. It allows pre-screening of plaque-containing tissue sections prior to immunostaining and processing for electron microscopy.

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Immunology and Infection

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
Fernando González Ibanez 1,2, Katherine Picard 1,2, Maude Bordeleau 1,3, Kaushik Sharma 1,2,4, Kanchan Bisht 1,2,4, Marie-Ève Tremblay 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.

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Immunology and Infection

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice
Kanchan Bisht 1,2, Kaushik Sharma 1,2, Ukpong B. Eyo 1,2
1Center for Brain Immunology and Glia (BIG), University of Virginia, 2Department of Neuroscience, University of Virginia

This protocol describes a chronic cranial window implantation technique that can be used for longitudinal imaging of neuro-glio-vascular structures, interactions, and function in both healthy and diseased conditions. It serves as a complementary alternative to the transcranial imaging approach that, while often preferred, possesses some critical limitations.

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