FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
5:30
Assessment of Neuronal Viability
7:08
Results: Double and Triple Stainings in CGN Culture
8:19
Conclusion
Transcrição
The overall goal of this procedure is to accurately measure cell viability in a cerebellar granule neuron culture. This method can help to answer key questions in the field of neuroscience and neuropharmacology. The main advantage of this techniqu
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This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.