Preparation of Dissection Instruments and Reagents
2:10
Isolation of Epithelial Cells from Rat Cauda Epididymides
6:09
Patch-clamp Experiment
8:32
Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
10:03
Conclusion
Transcrição
The overall goal of this procedure is to isolate epithelial cells from the rat epididymis and to measure the electrical properties on these primarily dissociated cells using whole-cell patch-clamp method. This method can help answer key questions
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We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.