Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

1:02

Treatment of Cells and Induction of DNA Damage

3:21

Cytocentrifugation, Fixation, and Permeabilization

5:35

Blocking and Primary Antibody Incubation

7:19

PLA Probes, Ligation, and Amplification

9:04

Mounting and Imaging

10:34

Results: The PLA Assay Detects Direct Protein-protein Interactions in Suspension Cell Cultures

12:03

Conclusion