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Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Mahnoush Bahjat¹, Timon A. Bloedjes¹, Amélie van der Veen¹, Guus de Wilde¹, Chiel Maas¹, Jeroen E. J. Guikema¹

¹Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.