Pre-amplification of Both Left and Right Junction DNA
8:15
Left- and Right-junction-specific Amplifications
10:23
Results: The IS Bidirectional Assay Improves Detection Rates and Sequence Quantification Accuracy
12:09
Conclusion
Transcrição
The overall goal of this procedure is to identify retroviral vector integration sites in the host genome and quantify the relative frequencies of clonal cell populations each sharing the same integration site. This method can help answer key quest
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This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets.