Set Up Fast Perfusion for Recording the Isolated Hippocampus
4:34
Extracellular Recording on In Vitro Hippocampal Theta Oscillations
7:57
Optogenetic Control in the Isolated Hippocampus
9:11
Results: Cell-type Specific Activity of Pyramidal Cell and PV Interneuron During Spontaneous Theta Oscillations in the Intact Hippocampal Preparation from a PV-TOM Mouse
10:19
Conclusion
TRANSCRIÇÃO
The overall goal of this protocol is to present procedures for extracting the whole hippocampal preparation and exploring the generation of rhythmic neuronal network using field, unitary and patch-clamp recordings as well as optogenetic stimulatio
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Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.