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Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Journal (Cancer Research)

Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Carli Stewart¹^,²^,³, Claudia Manriquez Roman¹^,⁴, Saad S. Kenderian¹^,⁵^,⁶

¹T Cell Engineering, Mayo Clinic, ²Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, ³Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, ⁴Center for Regenerative Biotherapeutics, Mayo Clinic, ⁵Division of Hematology, Mayo Clinic, ⁶Department of Immunology, Mayo Clinic

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Journal (Cancer Research) | Methods Collections

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Liang Hu¹, Robert Berahovich¹, Yanwei Huang¹, Shiming Zhang¹, Jinying Sun¹, Xianghong Liu¹, Hua Zhou¹, Shirley Xu¹, Haoqi Li¹, Vita Golubovskaya¹, Lijun Wu¹

¹ProMab Biotechnologies

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Journal (Cancer Research) | Methods Collections

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Maryna Somova¹, Marlene Grosse¹, Frank Schulze², Martin Burchardt¹, Pedro Caetano Pinto¹

¹Department of Urology, University Medicine Greifswald, ²Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Journal (Cancer Research) | Methods Collections

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

The Huong Chau¹, Naaz Bansal¹, Anastasia Chernykh¹, Rebeca Kawahara¹^,², Morten Thaysen-Andersen¹^,²

¹School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, ²Institute for Glyco-core Research (iGCORE), Nagoya University

Preclinical Positron Emission Tomography with Body Conforming Animal Molds for Cloud-Based Automated Image Analysis in Mice

Journal (Cancer Research)

Preclinical Positron Emission Tomography with Body Conforming Animal Molds for Cloud-Based Automated Image Analysis in Mice

Edward Cabral¹, Mary Katherine Montgomery¹, Meaghan Berg², Lisa Kathryn Manzuk², Anand Giddabasappa¹, Ziyue Karen Jiang¹

¹Drug Safety R&D, Discovery & Early Development, Pfizer, Inc., ²Comparative Medicine, Discovery & Early Development, Pfizer, Inc.

Staining Mouse Bone Marrow Cells with Hoechst to Detect Side Population Cells by Flow Cytometry

Journal (Cancer Research)

Staining Mouse Bone Marrow Cells with Hoechst to Detect Side Population Cells by Flow Cytometry

Fangfang Wang¹^,², Xinyu Zhai¹, Tingting Guo³, Hengyi Xiao², Jingcao Huang¹

¹Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ²The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, ³Precision Medicine Research Laboratory, West China Hospital, Sichuan University

Flow Cytometry of Hoechst-Stained Mouse Bone Marrow Cells for Effective Detection of Side Population Using High-Power 375 nm and 405 nm Lasers

Journal (Cancer Research)

Flow Cytometry of Hoechst-Stained Mouse Bone Marrow Cells for Effective Detection of Side Population Using High-Power 375 nm and 405 nm Lasers

Fangfang Wang¹^,², Xinyu Zhai¹, Tingting Guo³, Hengyi Xiao², Jingcao Huang¹

¹Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ²The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, ³Precision Medicine Research Laboratory, West China Hospital, Sichuan University

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Journal (Cancer Research) | Methods Collections

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Alan Halim¹, Sujan Kumar Mondal¹^,², Nasreen Al-Qadi¹, Elizabeth Kenyon¹^,², Keith MacRenaris³^,⁵, Thomas V. O’Halloran⁴^,⁵, Zdravka Medarova⁶, Anna Moore¹^,²

¹Precision Health Program, Michigan State University, ²Department of Radiology, College of Human Medicine, Michigan State University, ³Department of Microbiology, Genetics, & Immunology, College of Natural Science, Michigan State University, ⁴Department of Chemistry, College of Natural Science, Michigan State University, ⁵Elemental Health Institute, Michigan State University, ⁶Transcode Therapeutics Inc.

FISH for Gene Amplification Assessment in Formalin-Fixed Paraffin-Embedded Cancer Specimens

Journal (Cancer Research)

FISH for Gene Amplification Assessment in Formalin-Fixed Paraffin-Embedded Cancer Specimens

Collin Gilbreath¹, Yan Peng², Sihan Wu¹

¹Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, ²Department of Pathology, University of Texas Southwestern Medical Center

Effective Detection of Hoechst Side Population Cells by Flow Cytometry

Journal (Cancer Research) | Methods Collections

Effective Detection of Hoechst Side Population Cells by Flow Cytometry

Fangfang Wang¹^,², Xinyu Zhai¹, Tingting Guo³, Hengyi Xiao², Jingcao Huang¹

¹Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ²The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, ³Precision Medicine Research Laboratory, West China Hospital, Sichuan University

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Journal (Cancer Research)

Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

Frederic St-Denis-Bissonnette*¹^,², Melanie Kirkby*¹^,², Lisheng Wang²^,³^,⁴, Jessie R. Lavoie¹^,²

¹Biologic and Radiopharmaceutical Drugs Directorate, ²Department of Biochemistry, Microbiology and Immunology Institute, University of Ottawa, ³Centre for Infection, Immunity and Inflammation Institute, University of Ottawa, ⁴Regenerative Medicine Program, Ottawa Hospital Research Institute

Developing Organoid Models from Patient-Derived Melanoma Tissues for Enhanced Tumor Microenvironment Studies

Journal (Cancer Research)

Developing Organoid Models from Patient-Derived Melanoma Tissues for Enhanced Tumor Microenvironment Studies

Beatriz Goncalves¹, Shujing Liu¹, Xiaogang Zhang¹, Andrew Fan¹, Lingling Ou¹, Xiaowei Xu¹

¹Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania

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