Study of Protein-protein Interactions in Autophagy Research

1:25

Immunofluorescence

5:43

Representative Results for Immunofluorescence

6:16

Immunoprecipitation

6:38

Harvesting Cells

7:33

Protein Isolation

8:10

Bradford Assay

8:36

Antibody Coupling of Beads

9:26

Incubation of Total Cell Lysate with Beads

9:52

Elution of Proteins from Beads

10:18

Preparation of Input Controls

10:43

Denaturation of Proteins and Western Blot

13:06

Representative Results for Immunoprecipitation

13:29

Conclusion