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Sabanci University

4 ARTICLES PUBLISHED IN JoVE

Biology

Study of Protein-protein Interactions in Autophagy Research

Secil Erbil-Bilir¹, Nur M. Kocaturk¹, Melih Yayli², Devrim Gozuacik^1,3

¹Molecular Biology, Genetics and Bioengineering Program, Sabanci University, ²Information Center, Sabanci University, ³EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics, Sabanci University

Presented here are two antibody-based protein-protein interaction research techniques: immunofluorescence and immunoprecipitation. These techniques are suitable for studying physical interactions between proteins for the discovery of novel components of cellular signaling pathways and for understanding protein dynamics.

Biology

Diagonal Method to Measure Synergy Among Any Number of Drugs

Melike Cokol-Cakmak¹, Feray Bakan², Selim Cetiner¹, Murat Cokol^1,2,3

¹Faculty of Engineering and Natural Sciences, Sabanci University, ²Nanotechnology Research and Application Center, Sabanci University, ³Laboratory of Systems Pharmacology, Harvard Medical School

In this protocol, we describe how to make Loewe additivity-based drug interaction measurements for pairwise and three-way drug combinations.

Chemistry

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

Sina Abdolhosseinzadeh^1,2, Mirsajjad Mousavi³, Navid Haghmoradi³, Selmiye Alkan Gürsel^3,4

¹Institut des matériaux, École Polytechnique Fédérale de Lausanne (EPFL), ²Laboratory for Functional Polymers, Swiss Federal Laboratories for Materials Science and Technology (Empa), ³Faculty of Engineering and Natural Sciences, Sabanci University, ⁴SUNUM Nanotechnology Research Centre, Sabanci University

For a continuous and scalable synthesis of noble-metal-based nanocomposites, a novel photocatalytic reactor is developed and its structure, operation principles, and product quality optimization strategies are described.

Neuroscience

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Iskalen Cansu Topcu Okan^1,2, Mehri Ahmadian^1,2, Yesim Tutuncu^1,2, Halit Yusuf Altay^1,2, Cavit Agca^1,2

¹Molecular Biology, Genetics and Bioengineering Program, Sabanci University, ²Nanotechnology Research and Application Center (SUNUM), Sabanci University

This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.