Preparation of Plant Material and Hoyer's Solution
3:40
Ovule Separation and Clearance
5:18
Characterization of Embryo Patterning and Cell Identity via the Observation of Marker Protein Expression
7:46
Results: Embryogenesis Characterization in Arabidopsis
9:17
Conclusion
Transcrição
The overall goal of this method is to characterize the pattern formation of embryos during plant embryogenesis using the model organism. This method can help answer key questions in the plant matter field, such as embryogenesis in Arabidopsis. The
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This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.