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Capital Normal University

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Method for Characterizing Embryogenesis in Arabidopsis

¹College of Life Sciences, Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Capital Normal University, ²College of Life Science, Shanxi Normal University

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

Chemistry

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Xiao Wu^*1, Donglin Diao^*1, Zhangwei Lu^*1, Yu Han¹, Shi Xu², Xinhui Lou¹

¹Department of Chemistry, Capital Normal University, ²College of Life Sciences, Capital Normal University

A protocol for the in vitro selection and characterization of group-specific phthalic acid ester- binding DNA aptamers is presented. The application of the selected aptamer in an electrochemical aptasensor is also included.

Behavior

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

Tong Li^*1,2, Yujia Weng^*1,2, Daxiang Yang¹

¹Department of Zoology and Animal Physiology, College of Biological Sciences, China Agricultural University, ²College of Life Sciences, Capital Normal University

This protocol describes how to make a simple adult Drosophila behavior observation chamber, and how to take high-definition photographs/videos of the morphology or behavior of different types of fruit flies in the observation chamber through relatively simple and affordable methods.