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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

6.5K Views

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11:37 min

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June 18th, 2018

DOI :

10.3791/57474-v

June 18th, 2018

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6,507 Views

Frauke Hausburg^1,2*, Paula Müller^1,2*, Natalia Voronina^1*, Gustav Steinhoff^1,2, Robert David^1,2

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, ²Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University

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Keywords MicroRNA Transfer

Adult Bone Marrow derived Hematopoietic Stem Cells

Cell Engineering

Magnetic Targeting

Regenerative Medicine

Hematopoietic Stem Cell Modification

Mesenchymal Stem Cells

Bone Marrow Collection

Density Gradient Centrifugation

Mononuclear Cell Isolation

Magnetic Selection

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