Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
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Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
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Conclusion
Transcrição
This method can help answer key questions about glioblastoma tumors and how the extracellular matrix can facilitate treatment resistance. This technique enables modular construction of adaptable 3D cell culture platforms which can be used to cultu
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Here, we present a protocol for three-dimensional culture of patient-derived glioblastoma cells within orthogonally tunable biomaterials designed to mimic the brain matrix. This approach provides an in vitro, experimental platform that maintains many characteristics of in vivo glioblastoma cells typically lost in culture.