Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Data Analysis
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Results: Investigation of Protein-Protein Interactions at Cell-Cell Contacts by sFCCS
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Conclusion
Transcrição
This method can help to understand key questions in the cell-to-cell interaction field such as which receptors mediate adhesion or recognition in cell-to-cell contacts and whether specific ligands trigger their interactions. The main advantage of
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This protocol describes a fluorescence fluctuation spectroscopy-based approach to investigate interactions among proteins mediating cell-cell interactions, i.e. proteins localized in cell junctions, directly in living cells. We provide detailed guidelines on instrument calibration, data acquisition and analysis, including corrections to possible artefact sources.