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Abstract
Bioengineering
Detergent-Free Decellularization of the Human Pancreas for Soluble Extracellular Matrix (ECM) Production
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …Abstract
Islet transplantation (ITx) has the potential to become the standard of care in beta cell replacement medicine but its results remain inferior to those obtained with whole pancreas transplantation. The protocols currently used for human islet isolation are under scrutiny because they are based on the enzymatic digestion of the organ, whereby the pancreas is demolished, its connections to the body are lost and islets are irreversibly damaged. Islet damage is characterized by critical factors such as the destruction of the extracellular matrix (ECM), which represents the 3D framework of the islet niche and whose loss is incompatible with islet euphysiology. Researchers are proposing the use of ECM-based scaffolds derived from the mammalian pancreas to address this problem and ultimately improve islet viability, function, and lifespan. Currently available methods to obtain such scaffolds are harsh because they are largely detergent based. Thus, we propose a new, detergent-free method that creates less ECM damage and can preserve critical components of pancreatic ECM. The results show that the newly developed decellularization protocol allowed the achievement of complete DNA clearance while the ECM components were retained. The ECM obtained was tested for cytotoxicity and encapsulated with human pancreatic islets which showed a positive cellular behavior with insulin secretion when stimulated with glucose challenge. Collectively, we propose a new method for the decellularization of the human pancreas without the use of conventional ionic and non-ionic chemical detergents. This protocol and the ECM obtained with it could be of use for both in vitro and in vivo applications.
Erratum
Erratum: Detergent-Free Decellularization of the Human Pancreas for Soluble Extracellular Matrix (ECM) ProductionAn erratum was issued for: Detergent-Free Decellularization of the Human Pancreas for Soluble Extracellular Matrix (ECM) Production. The author list was updated.
The author list was updated from:
Riccardo Tamburrini1,2,3, Deborah Chaimov1,3, Amish Asthana1,3, Kevin Enck3, Sean M. Muir4, Justine Mariam Aziz5, Sandrine Lablanche6, Emily Tubbs6, Alice A. Tomei7,8, Mark Van Dyke9, Shay Soker3, Emmanuel C. Opara3, Giuseppe Orlando1,3
1Department of Surgery, Wake Forest Baptist Medical Center,
2Department of General Surgery, PhD Program in Experimental Medicine, University of Pavia,
3Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine,
4Wake Forest University College of Arts and Science,
5Wake Forest University School of Medicine,
6Laboratory of Fundamental and Applied Bioenergetics (LBFA), and Environmental and System Biology (BEeSy), Grenoble Alps University,
7Department of Biomedical Engineering, University of Miami,
8Diabetes Research Institute, University of Miami Miller School of Medicine,
9Department of Biomedical Engineering and Mechanics, School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University
to:
Riccardo Tamburrini1,2,3, Deborah Chaimov1,3, Amish Asthana1,3, Carlo Gazia3,4, Kevin Enck3, Sean M. Muir5, Justine Mariam Aziz6, Sandrine Lablanche7, Emily Tubbs7, Alice A. Tomei8,9, Mark Van Dyke10, Shay Soker3, Emmanuel C. Opara3, Giuseppe Orlando1,3
1Department of Surgery, Wake Forest Baptist Medical Center,
2Department of General Surgery, PhD Program in Experimental Medicine, University of Pavia,
3Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine,
4Department of Surgery, Tor Vergata University of Rome
5Wake Forest University College of Arts and Science,
6Wake Forest University School of Medicine,
7Laboratory of Fundamental and Applied Bioenergetics (LBFA), and Environmental and System Biology (BEeSy), Grenoble Alps University,
8Department of Biomedical Engineering, University of Miami,
9Diabetes Research Institute, University of Miami Miller School of Medicine,
10Department of Biomedical Engineering and Mechanics, School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University
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