Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
6:22
Results: Analysis of Cdr1-mGFPHis Isolated from Plasma Membranes of Saccharomyces cerevisiae
9:06
Conclusion
TRANSCRIÇÃO
The small scale plasma membrane isolation protocol and mGFPHis double tag, provide an economical, fast, reliable, and easy method to characterize membrane proteins in the eukaryotic model organism, Saccharomyces cerevisiae. The biggest advantage o
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This article presents a small-scale plasma membrane isolation protocol for the characterization of Candida albicans ABC (ATP-binding cassette) protein Cdr1, overexpressed in Saccharomyces cerevisiae. A protease-cleavable C-terminal mGFPHis double tag with a 16-residue linker between Cdr1 and the tag was designed to facilitate the purification and detergent-screening of Cdr1.