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Chulalongkorn University

5 ARTICLES PUBLISHED IN JoVE

Chemistry

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Zachary J. Kirsch¹, Blaise G. Arden¹, Richard W. Vachet^*1,2, Patanachai Limpikirati^*3

¹Department of Chemistry, University of Massachusetts Amherst, ²Molecular and Cellular Biology Program, University of Massachusetts Amherst, ³Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University

The experimental procedures for performing diethylpyrocarbonate-based covalent labeling with mass spectrometric detection are described. Diethylpyrocarbonate is simply mixed with the protein or protein complex of interest, leading to the modification of solvent accessible amino acid residues. The modified residues can be identified after proteolytic digestion and liquid chromatography/mass spectrometry analysis.

Bioengineering

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Suryo Kuncorojakti^*1,2, Watchareewan Rodprasert^*1, Quynh Dang Le^1,5, Thanaphum Osathanon^3,4, Prasit Pavasant^3,4, Chenphop Sawangmake^1,5,6

¹Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, ²Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, ³Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, ⁴Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, ⁵Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, ⁶Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University

This protocol presents a comparison between two different induction protocols for differentiating human dental pulp stem cells (hDPSCs) toward pancreatic lineages in vitro: the integrative protocol and the non-integrative protocol. The integrative protocol generates more insulin producing cells (IPCs).

Biology

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Golnoush Madani^*1, Erwin Lamping^*1, Hee Ji Lee¹, Masakazu Niimi^1,2, Alok K. Mitra³, Richard D. Cannon¹

¹Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, ²Department of Microbiology, Faculty of Medicine, Chulalongkorn University, ³School of Biological Sciences, University of Auckland

This article presents a small-scale plasma membrane isolation protocol for the characterization of Candida albicans ABC (ATP-binding cassette) protein Cdr1, overexpressed in Saccharomyces cerevisiae. A protease-cleavable C-terminal mGFPHis double tag with a 16-residue linker between Cdr1 and the tag was designed to facilitate the purification and detergent-screening of Cdr1.

Bioengineering

Superior Auto-Identification of Trypanosome Parasites by Using a Hybrid Deep-Learning Model

Veerayuth Kittichai¹, Morakot Kaewthamasorn², Suchansa Thanee², Thanyathep Sasisaowapak³, Kaung Myat Naing³, Rangsan Jomtarak⁴, Teerawat Tongloy³, Santhad Chuwongin³, Siridech Boonsang⁵

¹Faculty of Medicine, King Mongkut’s Institute of Technology Ladkrabang, ²Veterinary Parasitology Research Unit, Faculty of Veterinary Science, Chulalongkorn University, ³College of Advanced Manufacturing Innovation, King Mongkut’s Institute of Technology Ladkrabang, ⁴Faculty of Science and Technology, Suan Dusit University, ⁵Department of Electrical Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang

Worldwide medical blood parasites were automatically screened using simple steps on a low-code AI platform. The prospective diagnosis of blood films was improved by using an object detection and classification method in a hybrid deep learning model. The collaboration of active monitoring and well-trained models helps to identify hotspots of trypanosome transmission.

Chemistry

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Su Myat Win¹, Manee Saelee¹, Hla Myo^1,2, Nuntawat Khat-Udomkiri¹

¹School of Cosmetic Science, Mae Fah Laung University, ²College of Public Health Sciences, Chulalongkorn University

This protocol details the utilization of a polyol-based microwave-assisted extraction method for extracting phenolic compounds and natural antioxidants, representing a practical and environmentally sustainable approach to the development of ready-to-use extracts.