Preparation of Buffer, Tissue Sample, and Solution
2:15
Total Protein Extraction
3:42
SDS-PAGE and Western Blot Analysis
4:39
Ammonium Sulfate Precipitation
6:28
Ionic Exchange Chromatography with FPLC
8:14
Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
9:32
Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
11:24
Conclusion
Transcrição
Purification of recombinant protein from bacteria is a well-established method. However, several problems may occur when using these techniques. Having methods to extract protein from tissue is for sure appealing.
The main advantage with thi
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This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.