Small Scale Ganglioside Extraction and Partial Purification
3:35
Large Scale Ganglioside Extraction and Purification
5:52
Ganglioside Resolution and Staining
8:02
Results: Small and Large Scale Ganglioside Extraction and Partial Purification
9:18
Conclusion
TRANSCRIÇÃO
Gangliosides are expressed on all vertebrate cell surfaces and are especially abundant on nerve cells. They regulate cell signaling and cell-cell recognition. This protocol introduces ganglioside isolation and analysis.
The techniques were s
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Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.