Establishment and Passaging of Mutant Lines and Egg Cryopreservation and Resuscitatio
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Results: CRISPR/Cas9 Ribonuclease-Mediated Construction of Homozygous Locust Mutants
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Conclusion
TRANSCRIÇÃO
The migratory locust is an important agricultural test. This method provides an efficient and easy to yield protocol for the gene pressure methods of the It is a systematic and a detailed method of a CRISPR/CAS9 construction of homozygous locust m
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This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.