Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 TechnologyQiang Yan *1, Yingying He *2,3, Yang Yue 2,3, Luyao Jie 1, Tingmei Wen 2,3, Yumiao Zhao 2,3, Min Zhang 2, Tingting Zhang 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University
This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
