Results: Generating Electroporation-Assisted CRISPR-Cas9 Edits in Freshly Isolated Mouse Hepatocytes
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Conclusion
Transcrição
The main advantage of our technique is that it is an easy, fast, and reproducible approach for editing genes in primary mouse hepatocytes as a way to generate in vitro and in vivo disease models. Using our technique, it may be possible to knock do
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This protocol describes techniques for isolating primary mouse hepatocytes from the liver and electroporating CRISPR-Cas9 as ribonucleoproteins and mRNA to disrupt a therapeutic target gene associated with an inherited metabolic disease of the liver. The methods described result in high viability and high levels of gene modification after electroporation.