Results: Flow Cytometric-Based Assay to Study the Binding of an Aptamer and Cancer Cells
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Conclusion
Transcrição
This protocol will help researchers new to working with aptamers to be able to use them in their projects and also to understand the individual little steps that are required to complete the protocol. Aptamers are very versatile, and this protocol
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A necessary step in anticancer aptamer development is to test its binding to the target. We demonstrate a flow cytometric-based assay to study this binding, emphasizing the importance of including a negative control aptamer and cancer cells that are positive or negative for that particular protein.