<p class="jove_content">This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC).  Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.</p>

preparation-of-mouse-retinal-cryo-sections-for-immunohistochemistry

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Preparation of high-quality mouse eye sections for immunohistochemistry (IHC) is critical for assessing the retinal structure and function and for ...

Institution - University of Pennsylvania School of Veterinary Medicine

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