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To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Note: Ethylenediamine is toxic, caustic, flammable, and corrosive. This solution must be prepared and used in a fume hood.
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Prepare 200 mL of a 25% v/v solution of ethylenediamine in deionized water. Pour about 50 mL into a 250-mL beaker.
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Place a powder funnel in a 50-mL graduated cylinder and measure 50 mL of ethylenediamine.
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Transfer the ethylenediamine to a 200-mL volumetric flask. Add deionized water to the flask until the solution level reaches the line.
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Add a magnetic stir bar to the flask, then place it on a stir plate, and mix the solution for approximately one to two minutes until it is homogeneous. Turn off the stir plate when finished.
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Label a brown glass dispensing bottle ‘25% v/v ethylenediamine’. Remove the dispensing pump and use a funnel to transfer the ethylenediamine solution to the bottle.
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Reconnect the glass dispensing pump with a 20 mL capacity to the bottle.
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Retrieve the stir bar with a magnetic wand and set both on a paper towel to absorb the excess liquid. Store the bottle in a flammables cabinet until it is needed.
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When finished, thoroughly clean the volumetric flask, graduated cylinder, funnel, and stir bar, using your lab standard practices. Store the ethylenediamine in its approved storage location.