JoVE Logo
Faculty Resource Center

Sign In

Abstract

Protocol

Representative Results

Materials

Chemistry

Hydrolysis of an Ester

Published: Not Published

  1. Preparation of Laboratory

    Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.

    • Before you get started, put on a lab coat, safety glasses, and nitrile gloves. Ensure that the lab is equipped with enough lab coats, splash goggles, and gloves for all of the students.
    • Prepare 1 L of 3 M NaOH. Measure 120 g of NaOH pellets, and transfer the pellets to a 1-L volumetric flask. Add deionized water to the 1-L line. Place a stir bar in the flask and mix the solution until the pellets are fully dissolved.
    • Once the solution is homogeneous, transfer the solution to a glass bottle, cap it, and label it. Place the bottle in a central hood so that students have access to it.
    • Prepare 1 L of 1 M CaCl2 solution. Weigh 110.98 g of CaCl2, and transfer it to a 1-L volumetric flask. Fill the flask to the marked line with deionized water, add a stir bar, and mix the solution on a stir plate until the CaCl2 is fully dissolved.
    • Once the solution is homogeneous, transfer it to a clean glass bottle, cap it, and label it. Store the solution in a central hood.
    • Prepare at least 1 L of 3 M HCl, which will be used to neutralize any excess sodium hydroxide at the end of the experiment.
    • Obtain 5 silicone soap molds and leave them in a common area. Obtain at least five different fragrance oils and five powder soap dyes in a variety of colors, and set them near the molds.
    • Place a container of commercial liquid soap in a common area. Set coconut oil next to the balance making sure there are clean spatulas and weighing boats.
    • Set out the following glassware and equipment at each student lab station (we suggest that students work in pairs):
       1    Stir bar
       1    Hotplate
       1    Package of pH paper
       1    pH meter
       1    250-mL plastic squeeze bottle of DI water
       2    50-mL volumetric flasks
       2    100-mL glass graduated cylinders
       6    50-mL glass graduated cylinders
       1    50-mL beaker
       2    400-mL beaker
       2    Glass vials
       1    Glass stirring rod
       1    Small spoon
       1    Small spatula

Log in or to access full content. Learn more about your institution’s access to JoVE content here

What is a triglyceride?

A triglyceride is a molecule possessing three fatty acid molecules connected to a glycerol molecule through ester bonds.

What is the difference between a saturated fatty acid and an unsaturated fatty acid?

A saturated fatty acid has only single bonds in the carbon chain, whereas unsaturated fatty acids possess one or more double bonds in the carbon chain. The presence of double bonds a.......

Log in or to access full content. Learn more about your institution’s access to JoVE content here

  • Stirring hotplate 
    5
  • Stir bar
    5
  • Package of pH paper
    5
  • pH meter
    5
  • 250-mL wash bottle filled with DI water
    5
  • 50-mL volumetric flask
    10
  • 100-mL graduated cylinder
    10
  • 50-mL graduated cylinder
    30
  • 50-mL glass beaker
    5
  • 400-mL glass beaker
    10
  • 10-mL graduated cylinder
    5
  • 10-mL glass vials
    10
  • Labeling tape
    5 rolls
  • Glass stirring rod
    5
  • Small spoon/scoopula
    5
  • Spatula
    5
  • Silicone soap mold
    5
  • Small rubber stopper (3 cm)
    5
  • Commercial liquid soap (at least 1 bottle)
    1
  • Solid coconut oil
    750 g
  • Sodium hydroxide
    200 g
  • Calcium chloride
    150 g
  • 3 M Hydrochloric acid
    1 L
  • Powder soap dye (variety of colors)
    5
  • Fragrance oil (5 different kinds)
    5
  • Deionized water
    3 L
  • 1-L volumetric flask
    2
  • 1-L glass bottle with cap
    2
  • Glass funnel
    2
  • Large stir bar
    2
  • Top-loading balance (at least 1)
    -1
  • Analytical balance (at least 1)
    -1 Dependent on lab size
  • Lab wipes
    -1 Dependent on lab size
  • Weighing boats
    -1 Dependent on lab size

This article has been published

Video Coming Soon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved