A subscription to JoVE is required to view this content. Sign in or start your free trial.
Method Article
Mouse Dorsal Forebrain Explant Isolation
In This Article
Summary
This video demonstrates the protocol for isolating and culturing explants of the mouse forebrain from embyonic day 12 mice. Procedures for removal of the uterus, embryos from uterus, and dissection of embryos are given. In addition the methodology for transferring these explants onto specialized membranes on which they are cultured is demonstrated. The development of the forebrain can be studied in vitro using this preparations as well as changes in gene expression.
Abstract
Disclosures
The authors have nothing to disclose.
References
Access restricted. Please log in or start a trial to view this content.
Reprints and Permissions
Request permission to reuse the text or figures of this JoVE article
Request PermissionThis article has been published
Video Coming Soon
Copyright © 2025 MyJoVE Corporation. All rights reserved