A subscription to JoVE is required to view this content. Sign in or start your free trial.
Subcutaneous Transplantation Assay: A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model
In This Article
Overview
This video describes the method for transplantation of tumor cells from the melanoma-bearing donor zebrafish model to an immunocompromised recipient Casper zebrafish. It helps to visualize tumor engraftment and proliferation in the recipient fish.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Transplantation Assay
- Prepare recipient 2-3 month old Casper fish by irradiating with 25 Gy of gamma irradiation one day prior to transplantation. Allow the fish to recover in fish water.
- Euthanize a tumor-bearing fish according to institutional guidelines. Specifically, a fish is placed into a dish with 0.6 mg/mL tricaine until gill movement stops.
- Cut off the tumor and put it in a Petri dish with approximately 5 mL filter sterilized 0.9x PBS ....
Representative Results
Figure 1. Transplantation of melanoma cells. A) Uninjected Casper zebrafish. Scale bar = 500 μM. B) Subcutaneous transplantation site (arrowhead) on an irradiated Casper recipient immediately after injection with 50,000 melanoma cells. Scale bar = 200 μM. C) Irradiated Casper recipient showing.......
This article has been published
Video Coming Soon
Source: Iyengar, S. et al. Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model. J. Vis. Exp.(2012)
Copyright © 2025 MyJoVE Corporation. All rights reserved