eoe sub articles

EoE Sub Articles

1. Fungal culture for&#160;M.grisea
<ol>
	<li>Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains.</li>
	<li>Weigh 30 - 50 g of oatmeal, add this to 800 mL of distilled/deionized water (ddH2O), and boil the mixture for 30 min in the electric pot.</li>
	<li>Filter the boiled oatmeal juice into the beaker through a piece of gauze.</li>
	<li>Add 150 mL of tomato juice and 20 g of agar to the filtrate in the beaker and add ddH2O up to 1,000 mL.</li>
</ol>
...

establishment of rice plant leaf infection through fungal inoculation via wounding

Source: Zhang, M. et al., <a href="https://app.jove.com/v/57675">The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen&#160;Magnaporthe Grisea.</a>&#160;J. Vis. Exp.&#160;(2018)
This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

1. Fungal culture for&#160;M.grisea

	Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains.
	Weigh 30 - 50 g of oatmeal, add this to ...

Begin with a culture dish containing a rice leaf with small wounds, exposing the underlying epidermis.
Apply a moistening solution containing Tween-20, creating a conducive environment for fungal growth.
Place a mycelial plug of Magnaporthe grisea, consisting of conidiophores with conidia&#160; &#8212; reproductive fungal spores, over the wounded site.
Incubate the leaf in a humid chamber under photoperiod conditions to promote fungal growth.
During incubation, conidiophores release spores that adhere to the exposed epidermis, developing germ tubes.
The germ tube differentiates into a dome-shaped appressorium, which generates high turgor pressure, forming a sharp hypha &#8212; or penetration peg.
The penetration peg secretes cell wall-degrading enzymes, facilitating its entry into the epidermal cell and differentiation into invasive hyphae for nutrient acquisition.
These hyphae grow and spread within the epidermal cells through plasmodesmata&#160; &#8212; connecting channels between cells, and establishing the infection.
The fungus secretes toxins, causing cell necrosis and lesion formation near the wounded site on the leaves, indicating fungal infection.

establishment-rice-plant-leaf-infection-through-fungal-inoculation

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This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

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Protocol