JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.
1. Fungal culture for M.grisea
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Source: Zhang, M. et al., The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea. J. Vis. Exp. (2018)
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